Identification of Unknown Microbial Species
Order ID 53563633773 Type Essay Writer Level Masters Style APA Sources/References 4 Perfect Number of Pages to Order 5-10 Pages
Identification of Unknown Microbial Species
While it is important to correctly identify each of your organisms, the scientific process you use to reach this identification is also important. The report must follow the format for a traditional lab report. Therefore, it must include the following sections: Title page, Introduction, Materials and Methods, Results, Discussion and References. The written report is worth 32 points and the organism identification is worth 8 points (4 points for correctly identifying each organism).
Overall Format (4 points):
· Arial 12 point font, double spaced, 1 inch margins
· Entire report cannot be more than 8 pages in length. Points will be deducted for each additional page.
· No use of personal pronouns or quotations
· All organisms must be written in proper nomenclature
· Must use headings for each section (Introduction, Methods and Materials, Results, Discussion and References) and have a separate title page.
Title page: This should be the first page of your paper and should be centered. It must include:
· Name for your experiment. Choose a name that describes the main point of the article and dont simply put Identification of unknown bacteria. Get creative!
· Your name and unknown number
· Identity of both unknown organisms
Introduction: (5 points) This section should contain the background information for your paper.
· Provide a general explanation as to why we use Gram staining and biochemical testing to identify unknown bacteria.
· Include your statement of purpose and how it relates to the background information.
· What was the conclusion from your statement of purpose?
· Include the 6 possible organisms used for identification (must write full organism names)
· You should be citing references in this section in the correct format (see below for how to cite sources).
Methods and Materials: (4 points) This section should include the various overall techniques that you performed to reach your conclusion.
· Describe individual steps of how you preformed each procedure. I should be able to read your report and without any previous knowledge, be able to copy the technique. Try to be as concise as possible in your explanations.
· You must use references in this section. Reference the page number of the lab manual each of these techniques can be found on. For example: A Gram stain was performed on both microbes1(p47). Never write lab manual, you must write out the entire name (Introduction to Microbiology Laboratory Manual) of the manual and cite it correctly.
· Attach your final draft of your dichotomous key to this section and use it to describe your experimental plan (3 points). This key must have a proper figure heading (see below) and must be discussed in this section.
· DO NOT include any results in this section or your interpretation of the results.
Results: You must visually describe the appearance of all results. All results must be recorded in table format. You may choose to do multiple tables or a single table, but the information must be recorded in a table. This section must include:
· 5 colony characteristics (1 point).
· Appearance of each organism under the microscope after the Gram stain, including color and cell morphology (1 points).
· Outcomes (visual appearance) of each biochemical test (3 points).
· DO NOT interpret any of the results, leave that for the discussion.
· Your table(s) must have correct headings (see below).
· Do not cut tables off between pages.
Discussion: Discuss what knowledge was gained from your experiments and what the results from each technique mean about your organism.
· Explain isolation and pure culturing.
· Explain what the gram stain results mean about your organism. Why are Gram positive purple and Gram negatives pink? Be sure to talk about the cellular envelope differences here. (3 points).
· Discuss which organisms were you able to eliminate after each round of testing (1 point).
· Explain what the results of each biochemical test mean about the metabolism of the organisms (4 points). What sort of ability does the organism have based on your test result? Do they utilize any enzymes to achieve this ability? Be sure to include what end product is made in each one of the tests and what that end product tell us about the organism.
· DO NOT use quotes, this must be in your own words.
· The final statement in your discussion should be which 2 organisms you believe you were given.
References: You must provide a complete citation of the references that are used in the paper. All citations need to be in AMA format. (3 points).
· You need to cite the lab manual and any additional resources you use for the project.
· AMA format guidelines can be found in Blackboard under the Ex. 18 folder.
How to label figures and tables:
o Figure headings always go below the figure and table headings always go above the table.
o Label your figure and table with a number going in order of appearance in the paper. For example: If using 2 tables and then a figure, they would be labeled Table 1, Table 2 and Figure 1. Not Table 1, Table 2 and Figure 3.
o Each table and figure must have a description
Identification of Unknown Microbial Species
QUALITY OF RESPONSE NO RESPONSE POOR / UNSATISFACTORY SATISFACTORY GOOD EXCELLENT Content (worth a maximum of 50% of the total points) Zero points: Student failed to submit the final paper. 20 points out of 50: The essay illustrates poor understanding of the relevant material by failing to address or incorrectly addressing the relevant content; failing to identify or inaccurately explaining/defining key concepts/ideas; ignoring or incorrectly explaining key points/claims and the reasoning behind them; and/or incorrectly or inappropriately using terminology; and elements of the response are lacking. 30 points out of 50: The essay illustrates a rudimentary understanding of the relevant material by mentioning but not full explaining the relevant content; identifying some of the key concepts/ideas though failing to fully or accurately explain many of them; using terminology, though sometimes inaccurately or inappropriately; and/or incorporating some key claims/points but failing to explain the reasoning behind them or doing so inaccurately. Elements of the required response may also be lacking. 40 points out of 50: The essay illustrates solid understanding of the relevant material by correctly addressing most of the relevant content; identifying and explaining most of the key concepts/ideas; using correct terminology; explaining the reasoning behind most of the key points/claims; and/or where necessary or useful, substantiating some points with accurate examples. The answer is complete. 50 points: The essay illustrates exemplary understanding of the relevant material by thoroughly and correctly addressing the relevant content; identifying and explaining all of the key concepts/ideas; using correct terminology explaining the reasoning behind key points/claims and substantiating, as necessary/useful, points with several accurate and illuminating examples. No aspects of the required answer are missing. Use of Sources (worth a maximum of 20% of the total points). Zero points: Student failed to include citations and/or references. Or the student failed to submit a final paper. 5 out 20 points: Sources are seldom cited to support statements and/or format of citations are not recognizable as APA 6th Edition format. There are major errors in the formation of the references and citations. And/or there is a major reliance on highly questionable. The Student fails to provide an adequate synthesis of research collected for the paper. 10 out 20 points: References to scholarly sources are occasionally given; many statements seem unsubstantiated. Frequent errors in APA 6th Edition format, leaving the reader confused about the source of the information. There are significant errors of the formation in the references and citations. And/or there is a significant use of highly questionable sources. 15 out 20 points: Credible Scholarly sources are used effectively support claims and are, for the most part, clear and fairly represented. APA 6th Edition is used with only a few minor errors. There are minor errors in reference and/or citations. And/or there is some use of questionable sources. 20 points: Credible scholarly sources are used to give compelling evidence to support claims and are clearly and fairly represented. APA 6th Edition format is used accurately and consistently. The student uses above the maximum required references in the development of the assignment. Grammar (worth maximum of 20% of total points) Zero points: Student failed to submit the final paper. 5 points out of 20: The paper does not communicate ideas/points clearly due to inappropriate use of terminology and vague language; thoughts and sentences are disjointed or incomprehensible; organization lacking; and/or numerous grammatical, spelling/punctuation errors 10 points out 20: The paper is often unclear and difficult to follow due to some inappropriate terminology and/or vague language; ideas may be fragmented, wandering and/or repetitive; poor organization; and/or some grammatical, spelling, punctuation errors 15 points out of 20: The paper is mostly clear as a result of appropriate use of terminology and minimal vagueness; no tangents and no repetition; fairly good organization; almost perfect grammar, spelling, punctuation, and word usage. 20 points: The paper is clear, concise, and a pleasure to read as a result of appropriate and precise use of terminology; total coherence of thoughts and presentation and logical organization; and the essay is error free. Structure of the Paper (worth 10% of total points) Zero points: Student failed to submit the final paper. 3 points out of 10: Student needs to develop better formatting skills. The paper omits significant structural elements required for and APA 6th edition paper. Formatting of the paper has major flaws. The paper does not conform to APA 6th edition requirements whatsoever. 5 points out of 10: Appearance of final paper demonstrates the student’s limited ability to format the paper. There are significant errors in formatting and/or the total omission of major components of an APA 6th edition paper. They can include the omission of the cover page, abstract, and page numbers. Additionally the page has major formatting issues with spacing or paragraph formation. Font size might not conform to size requirements. The student also significantly writes too large or too short of and paper 7 points out of 10: Research paper presents an above-average use of formatting skills. The paper has slight errors within the paper. This can include small errors or omissions with the cover page, abstract, page number, and headers. There could be also slight formatting issues with the document spacing or the font Additionally the paper might slightly exceed or undershoot the specific number of required written pages for the assignment. 10 points: Student provides a high-caliber, formatted paper. This includes an APA 6th edition cover page, abstract, page number, headers and is double spaced in 12’ Times Roman Font. Additionally, the paper conforms to the specific number of required written pages and neither goes over or under the specified length of the paper.
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